IT 
Another female may now be cut up, and the eggs may be 
added to the contents of the same beaker, but if the females 
are large, and yield many eggs, it is not best to use more than 
one, for although there are enough male cells to fertilize a 
very great number of eggs, the eggs are heavier than water 
and soon sink to the bottom, and if they form a very thick 
layer, only those which lie near the surface have room to 
develop. 
The beaker should now be allowed to stand for about ten 
minutes, and in the meantime some of the eggs may be picked 
out with a dipping tube for examination under the micro- 
scope. In using the dipping tube, cover the large end with 
the tip of the finger, and run the small end down close to 
the bottom of the beaker, and then take the finger off the 
top, and as the water runs in at the bottom it will carry 
some of the eggs with it. When the tube is filled, place the 
finger on the top again, and draw it out of the water, and, 
holding it perpendicularly on the centre of a glass slide, and 
taking the finger off the top, allow a good sized drop to run 
out into the slide. 
If things are working properly, each egg should now have 
a number of male cells attached by their heads to its outer 
surface, with their tails radiating from it in all directions, as 
shown in Figure 51. 
It is not necessary that more than one male cell should 
fasten onto each egg, but they usually cover them in such 
numbers that the lashing of their tails causes the eggs to ro- 
tate and move through the water. 
As soon as all the eggs have male cells attached to them, it 
is necessary to get rid of the superfluous male fluid, for it 
would soon decay and pollute the water if it were allowed to 
remain, and if it is not drawn off from the eggs while they 
are at the bottom, it is almost impossible to remove it after 
the embryos have begun to swim, without loosing them as well. 
After a final stirring, the beaker should be allowed to stand 
for about five minutes, to allow the eggs to settle to the bot- 
tom, and the fluid above them should then be drawn off 
