INTESTINAL TRYPANOPLASMAS OF FISHES. 
177 
at the Aquarium at Naples for their kindness and help during 
my stay there, and I should also like to thank Mr. Elmhirst, 
the Director of the Marine Biological Station, for the great 
help he gave me in my work at Millport. 
Finally, I should like to thank Miss Robertson for her 
great kindness in giving me some preparations of the crops 
of leeches containing dividing Trypanoplasma cyprini, 
and thus enabling me to form some standard from which it 
was possible to judge how far these various intestinal flagel- 
lates of fishes are removed from the true blood Trypano- 
plasmas. I should like, also, to thank Professor Minchin for 
his kindness in allowing me to write up the results of this 
work in his department at the Lister. 
II. Methods. 
The methods adopted in this paper were the same as those 
detailed in my previous account of the division of Trypano- 
plasma congri, but in addition to the stains mentioned there 
I used Apathy^s hsematoxylin, followed by his Picro-ammoniak 
fuchsin. 
III. Trypanoplasma congri. 
I have decided in this part of my paper to give a text- 
figure of the stages that I have found of the division of 
Trypanoplasma congri, partly for the sake of comparison 
with the division stages of the other flagellates from the 
stomachs of fishes figured below, and partly to elucidate 
some abnormal division stages which I have found in Try- 
panoplasma congri. The normal stages of division of 
Trypanoplasma congri are shown in Text-fig. 1. It will 
be seen from this that — 
(1) The basal granule divides. This is followed imme- 
diately by a splitting of the anterior flagellum, and later 
by the splitting of the posterior flagellum and membrane. 
(2) The trophonucleus in the first stage enlarges, the intra- 
nuclear chromatin condensing on the karyosome. The 
trophonucleus assumes first a spindle and later a dumb-bell 
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