ST[TDTES IX THE EXPERIMENTAL ANALYSTS OF SEX. 283 
- Tlie normal males used for the experiments were in all cnses, 
•except three, hard-shelled males, which v/ere presumably about 
mid-way between two moults. 
In three experiments (marked with an asterisk in the 
table) soft -shelled crabs which had recently moulted wei-e 
chosen, and it will be at once observed what an exceedingly 
low percentage of fat and of glycogen these crabs possessed. 
It is clear, therefore, what a pronounced effect the moult has 
on the storage of food material in the crab’s liver, and the 
greater variability shown by the normal males as compared to 
the infected individuals as brought out by the table, is attri- 
butable in any case to a great extent to the fact that the 
normal males used were in various degrees of pi-oximity to a 
moult, whereas the infected individuals were all equally 
removed from it. 
Taking the total average percentages given at the bottom 
of the table, we find that the normal males on the average 
have more glycogen and less fat in the liver than infected 
•crabs when starved over a period of 24-532 hours. 
Taking the glycogen contents of the liver alone, it can be 
seen by consulting the second column of figui-es that starvation 
for periods up to 532 hours does not have any constant or 
perceptible effect in reducing the quantity of glycogen in the 
liver of normal male crabs. Thus, after 288 hours’ starvation 
the liver contained T17 per cent, glycogen, which is an average 
amount. Another point is that after only 24 hours’ starvation 
we may observe great variations in the glycogen content, viz. 
from T88 per cent, to *36 per cent. This is in keeping with 
histological results, where the variations in the quantity of 
glycogen in the liver appears to vary according to the period 
in the life-history of the crab, especially in relation to the 
moult. 
The infected crabs, whose liver-glycogen content is given in 
the fourth column, show a marked difference from the normal 
males. In the first place, a much more constant percentage 
■of glycogen is present after 24 and 48 hours’ starvation, 
varying only between T09 and *86, and still more conspicuous 
