MINUTES OF MEETING - March 6-7 
17 
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'The motion to amend the HV1CV nomenclature of the Guidelines to HV2 was 
denied by a vote of seven in favor, eleven opposed, arri three abstentions. 
A proposal to change the nomenclature from HV1CV to HV1 will appear in 
the Federal Register prior to the June RAC meeting . 
XIV. PROPOSED EXEMPTION FOR STREPTOCOCCUS MUTANS AND STREPTOCOCCUS SANGUIS 
Dr. Gottesman introduced the proposal of Dr. Francis Macrina of the 
Virginia Commonwealth University to include Streptococcus mutans and 
Streptococcus sanguis under the exemption category of Section I-E-4 of 
the Guidelines (tab 829, 843/9). She said this proposal requests the 
institution of a new sublist in Appendix A. She said it is a unique 
proposal in that Dr. Macrina proposes a one-way exemption. Dr. Campbell 
noted that Dr. Fredrickson, in the decision document accompanying the 1978 
(Sidelines, specifically endorsed one-way transfer lists in Appendix A. 
I 
Dr. Gottesman said the data supplied by Dr. Macrina demonstrate the 
transformation of Streptococcus sanguis by Streptococcus mutans ENA, but 
exchange from Strep tococcus sanguis into Streptococcus mutans has not been 
demonstrated. Streptococcus mutans to date appears to be nontrans formable . 
Dr. Gottesman said that the data showing unidirectional transfer is good, 
transformation occurs at reasonable frequencies and apparently occurs in 
vivo. Dr. Ahmed asked whether chromosomal gene transfer occurred. 
Dr. Gottesman said that it did. Dr. Gottesman moved that a new sublist 
of Appendix A be established for one-way transfer from Streptococcus 
mutans to Streptococcus sanguis . 
The RAC unaminously recommended acceptance of the motion by a vote of 
nineteen in favor, and none opposed. 
XV. PROPOSALS TO CIONE EXOTOXIN A PROTEIN OF PSEUDOMONAS AERUGINOSA 
Dr. Broadbent introduced a request (tab 830, 843/10) from Dr. C. W. Shuster 
of Case Western Reserve University to permit the cloning of the Pseudomonas 
aeruginosa exotoxin A gene in Escherichia coli K-12. Dr. Broadbent noted 
that this item had been considered at the December 6-7, 1979 RAC meeting. 
He said the RAC at that time recommended that the proposal be published 
in the Federal Register . In the period between the December and March 
RAC meetings, Drs. James Miller of the Olivers ity of Louisville and 
Dr. Stanley Falkow of the University of Washington submitted similar pro- 
posals. In support of his request. Dr. Stanley Falkcw submitted extensive 
documentation concerning Pseudomonas aeruginosa . Dr. Broadbent said 
Dr. Falkcw argued that exotoxin A is not a potent toxin in the class of 
botulinum or diptheria toxin and is not a primary determinant of disease. 
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