Federal Register / Vol. 45, No. 227 / Friday, November 21, 1980 / Notices 
77393 
with phages, plasmids and DNA from 
Class 2 agents. [f) 
III-A-2-a. Viruses of Eukaryotes 
(summary given in Table Eft see also 
exception given at asterisk at end of 
Appendix D). 
III-A-2-a-(l). DNA Viruses. 
UI-A-Z-a-flUa). Nontransforming 
viruses. 
III-A-2-a-<(l)-( a )-W Adeno- 
Associated Viruses, Minute Virus of 
Mice, Mouse Adenovirus (Strain FL, and 
Plant Viruses. (4Sf Pi physical 
containment -f an HVl host-vector shall 
be used for DNA recombinants 
produced with (i) the whole viral 
genome, (ii) snbgenamic DNA segments, 
or (iii) purified cDNA copies of viral 
mRNA. <571 
III-A-2-a-{lH a }-{^3- Hepatitis B, 
III-A-2-na-<lH®M^H tf 3- Fl physical 
containment -|- an HVl host-vector shall 
be used for purified subgenomic DNA 
segments. {36) 
III-A-2-a-(l)-(a}--t{2Hh). P2 physical 
containment + an HV2 host-vector, or 
P3 + HVl, shall be used for DNA 
recombinants produced with the whole 
viral genome or with subgenomic 
segments that have not been purified to 
the extent required in footnote 38. 
lU-A-2-a-{t)-[ai)-l2)-{d). P2 physical 
containment -f an HVl host-vector shall 
be used for DNA recombinants derived 
from purified cDNA copies of viral - 
mRNA.(JT) 
III-A-2-<a-(l}-[a)-{2). Other 
Nontraasf arming Member of Presently 
Classified Viral Families. (3$ 
III-A-2-a-(lJ-[a}-I;7)-{a). Pi physical 
containment -f an HVl host-vector shall 
be used for (i) DNA recombinants 
produced with purified subgenomic 
DNA(33) segments or pi) purified cDNA 
copies of viral mRNA.(373 
m-A-2-a-(lMaM3Hb3. PI physical 
containment + an HVl host-vector shall 
be used Tor DNA recombinants 
produced with the whole viTal genome 
or with subgenomic segments that have 
not been purified to the extent required 
in footnote 38. 
III-A-2-a-{l)-Ibh Transforming 
Viruses. (37A) 
III-A-2-a-(l)-(b)-(2). Herpes Saimiri, 
Herpes Ateles, and Epstein Barr 
Virus. (3^) 
III- A-2-a-flJ-^ b]-( 1 M °3 • PI physical 
containment -f an HVl host-vector shall 
be used for DNA recombinants 
produced with purified nontransforming 
subgenomic DNA segments.{369 
III-A-2-n-(l)-(bMfMbl. P2 physical 
containment + an HVl host-vector shall 
be used for (i) DNA recombinants 
produced with purified subgenomic 
DNA segments containing an entire 
transforming gene! 3#] or fri) purified 
cDNA copies of viral uiRNA.{3ri 
III— A— 2— a— (1)— (b)— (/)— (cr). P3 physical 
containment + an HVl host-vector, or 
P2 + HV2, shall be used for DNA 
recombinants produced with the whole 
viral genome or with subgenomic 
segments that have not been purified to 
the extent required in footnote 38. 
III-A-2-a-(lHbH2/ Other 
Transforming Members of Presently 
Classified Viral Families. (36) 
III-A-2-a-(lHbH2)-(o)- PI physical 
containment + an HVl host-vector shall 
be used for DNA recombinants 
produced with purified nontransforming 
subgenomic DNA segments^) 
!Il-A-:2^a-(l3-(b)-(2)-(h3. P2 physical 
containment + an HVl host-vector shall 
be used for (i) DNA recombinants 
produced with the whole viral genome, 
(ii) subgenomic DNA segments 
containing an entire transforming gene, 
(iii) purified cDNA copies of viral 
mRNA,(.?7) or (iv) subgenomic segments 
that have not been purified to the extent 
required in footnote 38. 
LII-A-2-a-f2). DNA Transcripts of 
RNA Viruses. 
III-A-2-a-(2)-( a) . Retroviruses. 
III-A-2-a-(2)-(a)-f7). Gibbon Ape, 
Woolly Monkey, Feline Leukemia and 
Feline Sarcoma Viruses.(3&) 
\\\-A-2-a-\Y)-[&)-[l)-{d). Pi physical 
containment + an HVl host-vector shall 
be used for DNA recombinants 
produced with purified nontransforming 
subgenomic DNA segments. [38) 
\l\-A-2-a-{Z)-[a)-[l)-{b'). P2 physical 
containment + an HVl host-vector shall 
be used for DNA reoombinants 
produoed with purified subgenomic 
DNA segmenla.{3<9] containing an entire 
transforming gene. 
III-A-2-a-(2)-i(a)-( .?)-(<:). P2 physical 
containment + an HV2 host-vector, or 
P3 -f HVl, shall be used fox DNA 
recombinants produced with (i) the 
whole viral genome, (ii) purified cDNA 
copies of viral mRNA,(373 or (iii) 
subgenomic segments that have not 
been purified to the extent required in 
footnote 38. 
III-A-2-a-(2)-(a)-(2). Other Members 
of the Family Retroviridiae.(36) 
III-A-2-a-(2)-(a)-(2)-(a). Pi physical 
containment + an HVl host-vector shall 
be used for DNA recombinants 
produced with purified nontransforming 
subgenomic DNA segments. [38) 
III-A-2-a-(2)-(a)-(2)-(h). P2 physical 
containment + an HVl host-vector shall 
be used for DNA recombinants 
produced with (i) subgenomic DNA 
segments containing an entire 
transforming gene, (ii) the whole viTal 
genome, or (iii) purified cDNA copies of 
viral mRNA, [37) or (iv) subgenomic 
segments that have not been purified to 
the extent required in footnote 38. 
III-A-2-a-(2)-(b). Negative Strand 
RNA Viruses. PI physical containment 
+ an HVl host-vector shall be used for 
DNA recombinants produced with (i) 
cDNA copies of the whole genome, (ii) 
subgenomic cDNA segments, or (iii) 
purified cDNA copies of viral mRNA. 
[37) 
III-A-2-a ~[2)-[c). Plus-Strand RNA 
Viruses. 
Ill— A— 2— a— (2)— fc)— (/). Types 1 and 2 
Sabin Poliovirus Vaccine Strains and 
Strain 17D (Theiler) of Yellow Fever 
Virus. Pi physical containment + an 
HVl host-vector shall be used for DNA 
recombinants produced with (i) cDNA 
copies of the whole viral genome, (ii) 
subgenomic cDNA segments, or (iii) 
purified cDNA copies of viral mRNA. 
[37) 
IlI-A-2-a-(2)-(c)-(2). Other Plus- 
Strand RNA Viruses Belonging to 
Presently Classified Viral Families. (36) 
III-A-2-a-(2Hc)-(2H0)- P1 physical 
containment + an HVl host-vector shall 
be used for DNA recombinants 
produced with purified subgenomic 
cDNA segments. [38) 
Table IN .Seoommended Containment tor Cloning of Viral DNA or cDNA in Certain HVl and HV2 Systems 
Specified in Appendix D 
[See text for full details] 
Type of viral DNA segment to be cloned 
Subgenomic [38] Genomic' cDNA 
from viral 
mRNA [37J 
Virus class Non- Segment Nonseg merited Segmented 
transforming containing genome genome 
segment an entire 
transforming 
gene 
\: 
Non transforming viruses: 
AAV, MVM, mouse adeno (strain FL).. 
? PI + HVl 
PI + HVl 
.... PI + HVl 
.... PI + HVl 
... PI + HVl 
.... PI +HVi1 
PI +HV1L38] 
.... P2 + HV2 
.... P2+ HVl 
Other 
or P3 + HV1 
.... P1+HV1 
.... PI -hMVI 
Transforming viruses; 
Herpes Saimiri, -H. Ateles araKEBV [39] 
P1+HVU38] P2 + HV1 
P2 + HV2 
.... P2 + HV1 
or P3 + HV1 
[ 12 ] 
