18 
MINUTES OF MEETING - June 5-6 * 
Dr. Novick asked if asporogenic Bacillus subtilis strains will persist in 
soil. Dr. Young replied that asporogenic Bacillus subtilis die more 
rapidly than sporogenic Bacillus subtilis but persist for a finite period. 
Dr. Gottesman moved to defer consideration of this proposal until 
additional data are available concerning transfer of DNA by transforma- 
tion out of ASB298. The RAC approved Dr. Gottesman' s motion by a vote of 
eleven in favor, two opposed, and one abstention. 
IX. PROPOSAL TO AMEND SECTION III-B-3 OF THE GUIDELINES 
Dr. Campbell introduced this proposal (tab 870, 881/6). He said that 
during discussions of hosts other than Escherichia coli at previous RAC 
meetings, the recommendation was made to permit cloning, in certain 
circumstances, in nonpathogenic bacterial hosts other than approved HV 
or EK hosts. After some discussion, a general provision was put into 
the Guidelines (Section III-B-3) to permit cloning of prokaryotic DNA in 
a non-HV certified prokaryote under P3 containment conditions provided 
neither the DNA cloned nor the host is pathogenic for man, animal, or 
plants. He said the P3 containment level was not meant to imply that 
all of these experiments actually require P3 containment. In order to 
clarify this point, he suggested amended language which parallels the 
Class 3 pathogen language evaluated earlier in the day. He said the 
proposed language would introduce one substantive change in Section III- 
B-3; under the amended language requests to lower containment levels for 
specific experiments in this class to less than P3 would still require 
review by the RAC but would no longer require Federal Register notice 
prior to RAC review. He moved approval of the proposal as published in 
the April 30, 1980, Federal Register . 
Dr. Krimsky asked Dr. Campbell if two nonpathogenic bacterial species 
might combine to produce a pathogen. Dr. Campbell said he did not be- 
lieve this would occur but said he could not predict a chance event. 
Dr. Gottesman said she thought the review by RAC afforded by the proposed 
language would permit RAC to consider such possibilities individually. 
The RAC then recommended approval of the preposed amendment of Section 
III-B-3 by a vote of thirteen in favor, none opposed, and four abstentions 
X. PROPOSAL TO DELETE LANGUAGE REQUIRING USE OF CaMV MUTANTS LACKING TOE 
APHID TRANSMISSION FACTOR FROM SECTION III-C-3 OF THE GUIDELINES; REQUEST 
FOR PERMISSION TO TRANSFER DNA FRAGMENTS FROM APHID TRANSMISSIBLE TO 
APHID NONTRANSMISSIBLE CaMV 
Dr. Brill introduced the request (tab 869, 881/7) of Dr. Robert Shepherd 
of the University of California at Davis. He said Dr. Shepherd requested 
deletion, from Section III-C-3 of the Guidelines, of language restricting 
the use of Cauliflower Mosaic Virus (CaMV) as vectors to mutants lacking 
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