22 
MINUTES OF MEETING - June 5-6 
I 
XV. PROPOSED FOOTNOTE REGARDING VARIOLA AND WHITEPOX VIRUSES 
— 
Dr. Maas introduced the proposal (tab 881/15) to footnote any mention in 
the Guidelines of alastrim, smallpox, and whitepox viruses. He said the 
purpose of the footnote was to call attention to World Health Organization 
policy regarding these viruses and worldwide efforts at eradication. 
I 
Dr. Goldstein asked how smallpox is currently classified by the CDC. 
Dr. Bems replied that the 1974 CDC classification, compiled before small- 
pox was eradicated, lists smallpox as Class 3 when used _in vitro and 
Class 4 when used in vivo . Work with alastrim, smallpox, and whitepox 
is now restricted Ey the World Health Organization to a few research and 
storage centers scattered throughout the world. Dr. Milewski said that 
the proposed language had been composed by the CDC. 
Dr. Maas moved acceptance of the language as it appeared in the Federal 
Register . The RAC accepted this recommendation by a vote of seventeen 
in favor, and none opposed. 
XVI. PROTOCOLS REQUIRING ASSIGNMENT OF CONTAINMENT LEVELS 
A. Request for Permission to Transform Saccharomycopsis lipolytica with 
Escherichia coli/Saccharcmyces cerevisiae Hybrid Plasmids 
B. 
Dr. Broadbent introduced the request (tab 874, 881/5) of Dr. David 
Ogrydziak of University of California at Davis for permission to 
transform Saccharomycops i s lipolytica with Escherichia col i/Sacchar- 
omyces cerevisiae hybrid plasmids. Er. Broadbent said Saccharomycopsis 
lipolytica is non-pathogen ic and does not colonize animals. In the 
absence of pathogenicity or toxin production, Dr. Broadbent recommended 
PI containment for the proposed experiments. Dr. Young concurred. 
He said Saccharomycops is lipolytica , a soil organism, has been used 
industrially for fermentation and the production of citric acid. 
i 
I 
Dr. Campbell asked Dr. Broadbent to clarify whether he wished to 
recommend PI containment for Dr. Ogrydziak' s proposed experiments or 
whether he was recommending SaccharomYcops is as a HV1 host-vector sys- 
tem. Dr. Broadbent replied that he wished to reccnmend PI containment 
for the specified experiments. He moved acceptance of PI containment 
conditions. The RAC accepted the motion by a vote of fifteen in 
favor, none opposed and one abstention. 
Request for Use of Conjugative Plasmids and Transducing Phages in an 
Escherichia ooli K-12 host 
1. Dr. Campbell introduced the proposal (tab 879, 881/19) of 
Dr. A. I. Bukhari of the Cold Spring Harbor Laboratory. He said 
Dr. Bukhari wishes to employ conjugative plasmids or transducing 
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