MINUTES OF MEETING - June 5-6 
23 
phages in recombinant DNA experiments with E^_ coli when a small 
fragment of Adenovirus 2 DNA has been used as linker DNA. He 
said Dr. Bukhari argues that this experiment is essentially 
self-cloning and said he tended to agree. Dr. Campbell moved 
approval of Dr. Bukhari's preposed experiments. 
Dr. Goldstein asked which Adenovirus 2 DNA fragment was to be 
used as the linker. Dr. Baltimore replied that the fragment 
comes from a region far from the transforming region. 
The question was called and the RAC recommended approval of 
Dr. Bukhari's request by a vote of fourteen in favor, none 
opposed, and one abstention. 
2. Dr. Gottesman initiated discussion of a second proposal (tab 893) 
submitted by Dr. Bukhari. It was noted that this second proposal 
had been received after the publication deadline and had not 
appeared in the Federal Register . Therefore, no action could be 
taken at the June 6-7, 1980 meeting. 
Dr. Gottesmn said Dr. Bukhari proposed to amend the definition 
of EKl to permit transient use of conjugation-proficient plasmids 
or generalized transducing phages; i.e., the host-component could 
contain these vectors but only if they are used as vehicles to 
transfer recombinant DNA molecules from one strain to another and 
all the intermediate strains and transducing lysates are destroyed. 
She said her general inclination would be to reject Dr. Bukhari's 
proposal and to continue to review specific requests on a case-by- 
case basis. Others agreed. 
Dr. Setlow suggested that Dr. Campbell discuss this request and the 
sentiment of the committee with Dr. Bukhari. 
C. Request for Exception to a Prohibition to Clone DNA from a CPC Class 3 
Agent 
Dr. Maas introduced the request (tab 880, 881/20, 890) of Dr. Sebastiao 
Baeta Henriques of the Institute de Ciencias Biologicas, Belo Horizonte, 
Brazil. Dr. Maas said Dr. Henriques requests an exception to a prohi- 
bition in order to clone, in Escherichia coli K-12, DNA from Shistoscma 
mansonii , a CDC Class 3 etiological agent. He said Dr. Henriques 
hopes to develop a vaccine against shistosomiasis through the use of 
recombinant DNA techniques. Dr. Maas said Dr. Henriques' proposal 
had been discussed briefly at the March 6-7, 1980 meeting but had not 
been published in the January 31, 1980, Federal Register and could not 
be acted on in March. 
fl23J 
