MINUTES OF MEETING - June 5-6 
33 
animals or plants from these experiments. He felt the experiment to be a 
very important one and moved acceptance of Dr. Davis' proposal. Dr. Zaitlin 
concurred, although he noted that Dr. Davis had not indicated the method 
by which the com will be transformed nor the vector to be used. 
Dr. Novick said that agricultural plants do not have the same potential 
for dissemination as do wild plants. Dr. Scandalios said that com gene- 
tic material can be dispersed through the pollen. Dr. Tolin noted that 
dispersion can be prevented by detasseling. She said that the shoots or 
ear can also be bagged, preventing fertilization and seed formation. She 
added that harvesting the ears before maturity would also prevent disper- 
sal, as only mature seeds can propagate. Dr. Baltimore noted that seed 
companies must continually deal with the problem of cross-fertilization 
of seed grain, and appear to control crosspollenization quite effectively. 
Dr. Broad bent pointed out that the experiments were to be conducted in 
an area where no substantial amounts of com are grown. 
The RAC recommended approval of this proposal by a vote of eleven in 
favor, none opposed, and five abstentions. 
XXIII. PROPOSAL TO AMEND SECTION III-C-7-b OF THE GUIDELINES 
Dr. Brill introduced the proposal (tab 889, 881/14) made by Dr. Setlcw 
bo include in the Guidelines a provision to permit plant ENA cloned in 
Saccharomyces cerevisiae to be subsequently returned to the plant. 
Dr. Setlow had suggested that the first sentence of Section III-C-7-b be 
amended to read as follows: 
"III-C-7-b. ENA from any nonprohibited source (Section I-D) which 
has been cloned and propagated in EL_ coli or S^ cerevisiae under 
appropriate containment conditions, may be transferred with the 
E. coli or S^_ cerevisiae vector used for cloning to any higher plant 
organisms (Angios perms or Gymnosperms) and propagated under condi- 
tions of physical containment comparable to PI and appropriate to 
the organism under study [2A]." 
Dr. Baltimore pointed out that Section III-C-7-b already applies to ENA 
cloned in Escherichia coli K-12 host-vector systems. 
Dr. Zaitlin moved acceptance of the proposal. The RAC recommended approval 
by a vote of twelve in favor, none opposed, and six abstentions. 
[ 133 ] 
