TABLE OF CONTENTS 
Part 
Page 
XVII. 
XVIII 
XIX. . 
XX. . . 
XXI. . 
XXII. 
XXIII 
XXIV. 
XXV. . 
Proposed Containment for Cloning Among Members 
of the Actincmycetes Group 20 
Protocol Requiring Assignment of Containment Levels 22 
Proposed Amendment of Section IV-E-2 22 
Minor Modifications of Large-Scale Protocols 22 
Request for Certif ication of HV1 Bacillus subtil is 
Host-Vector System 23 
Protocols Requiring Assignment of Containment Levels 24 
A. Requests for permission to transform Chlamydomonas 
reinhardi with EL coli/S, cerevisiae plasmids 24 
B. Request for permission to transform Candida albicans 
with EL coli/S. cerevisiae hybrid plasmids 24 
Institutional Biosafety Committee (IBC) Survey 
(Continued) 25 
Discussion of Incident at University of California, 
San Diego i 25 
Protocols Requiring Assignment of Containment Levels 
(Continued) 26 
C. Proposal to introduce genes cloned in E. coli K-12 
into Arabidopsis plants through the use of Agro- 
bacterium tumefaciens carrying an EL_ coli/Ti 
hybrid plasmid vector 26 
D. Request for consideration of appropriate contain- 
ment for experiments involving Zymomonas mobilis Jll 
E. Request to clone Sch izosaccharomy ces pombe DNA in 
Schizosaccharorryces pombe 28 
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