16 
By a vote of fifteen in favor, none opposed, and two abstentions the RAC 
recommended the following language: 
" Streptomyces coeli color , S. aureofaciens , S . rimosus , S . gris- 
eus , S . cyaneus , and venezuelae can 5e used as hosts for the 
cloning of ENA derived from subtilis, Eh coli K-12 or from 
S . aureus vectors that have been approved for use in B. subtilis , 
under P2 conditions, using as vectors any nonconjugative plasmids 
indigenous to these Streptomyces species or able to replicate in 
these hosts by natural biological mechanisms." 
Dr. Novick asked if this modified language might restrict researchers 
already working under Appendix E. Dr. Taylor of Smith Kline and French 
Company replied that several investigators are developing conjugative 
plasmid vectors under this section of Appendix E. Dr. Novick suggested 
that more concrete language on this issue might be considered at the 
next meeting. 
(Executive Secretary’s note: The Director, NIH, in his decision deleted 
the requirement for use of "nonconjugative plasmids." The reasons for 
this decision are presented in the Federal Register of November 21, 1980). 
XV. REQUEST FOR EXEMPTION OF STREPTOCOCCUS SANGUIS AND STREPTOCOCCUS PNEUMONIAE 
UNDER SECTION I-E-4 
Dr. Gottesman presented the proposal (tabs 919, 927, 931/10) from 
Dr. Walter R. Guild, Duke University. Dr. Guild proposed that Streptococcus 
sanguis and Streptococcus pneumoniae be added to Appendix A of the Guide- 
lines. Et. Gottesman said the evidence for natural exchange of genetic 
material between the two organisms in both directions is good. She 
moved that these organisms be added to Appendix A as a new sublist. 
By a vote of sixteen in favor, none opposed, and one abstention, the 
committee adopted the proposal. 
XVI . PROPOSED PROCEDURES FOR REVIEW OF LARGE-SCALE APPLICATIONS ( Continued ) 
Mr. Thornton reminded the conmittee that it had earlier (Section V) adopted 
language defining the RAC's role in the review of large-scale proposals 
and that Dr. Krimsky (Section VIII) had proposed a new RAC subcommittee 
be involved in the review. 
Dr. Krimsky moved the following language: 
"An industrial review subcommittee of the RAC shall be esta- 
blished with the responsibility for advising the Director 
[ 175 ] 
