24 
By a vote of eighteen in favor, and none opposed, the RAC recommended 
that plasmid pAB124, isolated from Bacillus stearothermophilus , be 
certified for use with HV1 certified Bacillus subtilis hosts, as an HV1 
host-vector system. 
XXII. PROTOCOLS REQUIRING ASSIGNMENT OF CONTAINMENT LEVELS 
A. Requests for permission to transform Chlamydomonas reinhardi with 
E. coli/S. cerevisiae plasmids 
Dr. Brill introduced the requests (tabs 910, 911, 931/1) from 
Dr. John Carbon, Lhiversity of California, Santa Barbara, and 
Dr. Stephen Howell, University of California, San Diego. He said 
these investigators request permission to use the unicellular 
flagellate Ch 1 amydamonas reinhardi , under P2 physical containment, 
to clone defined DNA segments derived from EL coli and S^ cerevisiae 
using EL coli/S. cerevisiae hybrid vectors. 
Dr. Brill said Chlamydomonas reinhardi is the most studied green alga, 
and has not been demonstrated to be a pathogen or to produce toxins. 
He moved approval of the proposal. Dr. Scandal ios concurred and added 
that the organism does not freely exchange ENA with other species. 
By a vote of sixteen in favor, none opposed, and one abstention, the 
RAC adopted the motion to approve the requests. 
B. Request for Permission to Transform Candida albicans with E. coli/ 
S. cerevisiae Hybrid Plasmids 
Dr. Maas introduced the requests (tabs 912, 913, 928, 931/2) from 
Dr. P. T. Magee, Michigan State University and Dr. W. LaJean Chaffin, 
Texas Tech Lhiversity. The investigators had requested considera- 
tion of the appropriate containment level for the return of Candida 
albicans ENA to the host of origin. The Candida albicans DNA will 
be cloned in an EK1 EL coli K-12 or in a laboratory strain £L cerevisiae 
employing a hybrid EL coli/ S. cerevisiae plasmid vector, or the 
S. cerevisiae 2 micron plasmid. 
Dr. Maas said Candida albicans is an opportunistic pathogen and is 
classified in the proposed revised CDC Biosafety Guidelines as a Class 
2 etiological agent. He said the investigators hope to analyze the 
genetics of the organism using recombinant ENA techniques. Ultimately 
they hope to elucidate the basis of C^ albicans pathogenicity'. 
[ 183 ] 
