27 
(2) Arabidopsis DNA will be introduced into the coli/ Ti hybrid 
plasmid and cloned in coli K-12. 
(3) The thiamine gene of E^_ coli K-12 will be introduced into the 
E. coli/Ti vector carrying Arabido psis DNA, and cloned in _E^_ coli 
K-12. 
(4) The hybrid plasmid into which Arabidopsis DNA and the thiamine 
gene have been ligated will be transformed into Agrobacterium 
tumef aciens . 
(5) Agrobacterium tumefaciens will be used to introduce the E^ coli/ 
Ti plasmid vector carrying the E^ coli thiamine gene and Arabidop- 
sis ENA into Arabidopsis plants . 
Steps (1) (2) and (3) are covered by Section III-O of the Guidelines 
and may be performed under PI containment. Steps (4) and (5) are 
covered under entry four of Appendix E and may be conducted under P3 
containment. Dr. Merlo requested permission to perform steps (4) and 
(5) at PI or P2 containment. 
Dr. Brill noted that Arabidopsis plants are easily contained. He said 
Agrobacterium tumefaciens is not a serious plant pathogen, as it only 
invades injured tissues. He moved that the investigator be permitted 
to perform steps (4) and (5) under PI containment conditions. 
Dr. Scandal ios concurred. 
Dr. Goldstein moved to amend Dr. Brill's motion to require P2 physical 
containment for steps (4) and (5). Dr. Brill did not accept the amend- 
ment. By a vote of four in favor, nine opposed, and three abstentions, 
the amendment was not accepted by RAC. 
Mr. Thornton then called the vote on Dr. Brill's motion. By a vote 
of twelve in favor, two opposed and three abstentions, the committee 
accepted the motion to set containment at PI for steps (4) and (5) 
of the proposed protocol. 
D. Request for Consideration of Appropriate Containment for Experiments 
Involving Zymomonas mob ills 
Dr. Maas introduced the request (tabs 922, 931/13) from Drs. B. S. 
Montencourt and D. E. Eveleigh, Rutgers University, to introduce 
into Zymomonas mobilis , DNA from non-pa thogenic Pseudomonas fe trains 
that had been cloned in E. coli K-12. 
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