VII . PHYSICAL CONTAINMENT FOR LARGE-SCALE USES OF ORGANISMS CONTAINING 
RECOMBINANT DNA MOLECULES 
This part of the NIH Guidelines specifies the laboratory practices, contain- 
ment equipment and special facility design for large-scale (greater than 10 liters 
of culture) research and production involving organisms containing recombinant DNA 
molecules. It shall apply to all large-scale research and production activities 
approved by the Director, NIH in accordance with Sections IV-E- 1-b- ( 1 )- (e) and 
IV-E-l-b-(3)-(d) of the NIH Guidelines. 
All provisions of the NIH Guidelines shall apply to large-scale research 
and production activities with the following modifications: 
° Part VII shall replace Section II-B when quantities in excess 
of 10 liters of culture are involved in research or production. 
0 The institution shall appoint a biological safety officer 
if it engages in large-scale research or production activi- 
ties involving organisms containing recombinant DNA molecules. 
The duties of the biological safety officer shall include 
those specified in Section IV-D-4. 
VII-A. Selection of Physical Containment Levels . The selection <?f the 
physical containment level required for recombinant DNA research or pro- 
duction involving more than 10 liters of culture is based on the physical 
containment levels established in Sections II and III of these guidelines. 
For purposes of large-scale research or production, two physical contain- 
ment levels are established. These are referred to as P2-LS and P3-LS. 
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