3 
VII-B-l-f . Animals shall not be permitted in the production area. 
VII-B-l-g. The wearing of protective garments or uniforms is required. 
Such clothing shall not be worn outside of the production area. Clothing 
shall be decontaminated before laundering. 
VII-B-l-h. Access doors to laboratories and rooms housing production 
operations shall be kept closed while work is in progress. 
VII-B-l-i. Mechanical pipetting devices shall be used; pipetting by 
mouth is prohibited. 
VII-B-l-j. Precautions shall be taken in the conduct of all procedures 
to minimize aerosol formation. 
VII-B-l-k. Use of hypodermic needles shall be avoided when alternative 
methods are available. 
VII-B-1-1. Bulk volumes or concentrates of inactivated organisms con- 
taining recombinant DNA molecules shall be contained in durable leak proof 
containers for transport outside the production area. Such materials may be 
transferred to other facilities provided that inactivation of the organisms 
has been demonstrated. 
VII-B-l-m. All contaminated wastes shall either be steam-sterilized 
(autoclaved) before disposal or incinerated. Contaminated equipment and 
materials shall be steam-sterilized or disinfected prior to processing for 
reuse . 
VII-B-l-n. Wastes and contaminated equipment or materials that are to 
be steam-sterilized, disinfected, or incinerated at a location outside the 
production area shall be transported in a durable leak proof container which 
is sealed prior to exit from the production area. 
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