6 
in all documentation relating to use of this equipment for the production 
of organisms containing recombinant DNA molecules. 
VII-B-3. Special Facility Design . 
VII-B-3-a. An autoclave for sterilization of wastes and contaminated 
materials shall be available in the same building used for the production 
of organisms containing recombinant DNA molecules. 
VII-B-3-b. Equipment used for the propagation, inactivation, or other 
processing of volumes greater than 10 liters of organisms containing recombi- 
nant DNA molecules shall be isolated by a curb or similar architectural 
feature of such size as to contain the total liquid volume contained in the 
equipment. 
VII-B-3-c. Floor drains serving a curbed or similar area shall be 
sealed during periods when production is in progress. 
VII-B-3-d. The floor shall be readily cleanable and penetrations through 
the floor shall be sealed to preclude passage of liquids. 
VII-C. P3-LS Level . 
VII-C-1. Laboratory Practices . 
*VII-C-l-a . Personnel entry into the production area shall be through 
an air lock, or other controlled access area. 
*vn-C-l-b. Persons entering the production area shall exchange their 
personal clothing for work garments such as jumpsuits, or pants and shirts, 
plus head cover, and shoes. On exit from the production area the protective 
clothing may be stored in a locker separate from that used for personal 
*Not required for P2-LS. 
[ 235 ] 
