8 
VII-C-1-k. Access doors to laboratories and rooms housing production 
operations shall be kept closed while work is in progress. 
VII-C-1-1. Mechanical pipetting devices shall be used; pipetting by 
mouth is prohibited. 
*vn-C-l-m. Procedures which may result in the release of aerosols 
shall be avoided or properly contained to prevent exposure of persons 
and contamination of the environment. 
VII-C-1-n. Use of hypodermic needles shall be avoided when alterna- 
tive methods are available. 
VII-C-l-o. Bulk volumes or concentrates of inactivated organisms 
containing recombinant DNA molecules shall be contained in durable leak 
proof containers for transport outside the production area. Such materials 
may be transferred to other facilities provided that inactivation of the 
organisms has been demonstrated. 
*VII-C-l-p. All contaminated wastes shall either be steam-sterilized 
(autoclaved) within the production area before disposal or incinerated. 
Contaminated equipment and materials shall be steam-sterilized or disin- 
fected within the production area prior to processing for reuse. 
VII-C-1-q. Contaminated wastes to be disposed of by incineration 
shall be placed in a leak proof container which is sealed prior to 
exit from the production area. 
VII-C-1-r. All work surfaces shall be disinfected following completion 
of procedures involving the handling of organisms containing recombinant DNA 
molecules . 
*Not required for P2-LS. 
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