Page 37 of Attachment H 
TABLE 4 
L -ASPARAGINASE - BIOLOGICAL TESTS 
CT-1570-8A 
(132 1 .11. /mg) 
Pyrogens: 
Passes U.S.P. requirements at dose of 200 I.U./kg. 
Mean temperature rise was 0.16°C in five rabbits. 
1000 I.U./kg. Mean temperature rise was 0.33°C in 
three rabbits. 
Safety Tests: 
4500 I.U./kg injected I.V. in mice caused no 
deaths over a 7-day observation period. 
Depressor Activity: 
200 I.U./kg contain less depressor activity 
than 0.1 microgram histamine base. 
Endotoxin: 
No evidence of endotoxin by carbon clearance 
test at i.p. dose of 5000 I.U./kg. 
(Sensitivity of test: 0.01 microgram/mouse.) 
[ 473 ] 
