Page 40 of Attachment H 
FOR THE RECORD 
2 . 
Personnel engaged in work involving manipulation of organisms containing 
recombinant DNA molecules are provided medical surveillance. Prior to 
assignment these personnel are provided a physical examination which 
includes a variety of clinic procedures. Periodic examination is not 
provided other than that associated with job associated illness or injury 
or when an employee has been absent from work for 5 or more days due to 
non- job associated illness or injury. Personnel absent less than 5 days 
because of non-job associated illness or injury are not required to report 
to the medical service prior to re-entry. A physical examination is pro- 
vided for all personnel on termination of employment. 
P3 Laboratories 
There are two laboratories (at separate locations) which are utilized for 
research involving recombinant DNA. 
One of the laboratories is a single room which is isolated by an air lock 
which is fitted with electrically interlocked door3. The laboratory is 
equipped with a Class II, Type B biological safety cabinet. An autoclave 
is located in a space which is accessed from the laboratory room. The 
laboratory is served by an independent ventilation system and is maintained 
negative to the corridor. Persons entering the laboratory wear protective 
clothing which is donned and removed in the air lock. 
The second laboratory is a complex of rooms, i.e. , two biochemistry labo- 
ratory rooms, a biological laboratory which serves as the P3 laboratory 
and a cold room which are served by a common corridor. Access to the area 
is controlled by key operated doors at each end of the corridor. The venti- 
lation for the area is adjusted so that the corridor is negative to the 
balance of the building. Internally the biological laboratory is negative 
to the controlled-access corridor. The biological laboratory is equipped 
with two Class II, Type A biological safety cabinets. The air balance is 
monitored through both local and central alarm systems. The exhaust air 
is passaged through a HEPA filter prior to discharge. The autoclave 
serving the complex is located on the same floor but is outside the 
controlled access area. The sinks in the biological laboratory are equipped 
with foot-operated valves. 
Recombinant DNA Production Facility 
The facility employed for the conduct of studies for the development of 
production methods is a single room. Two 150 liter stainless steel 
fermenters are housed in the room. The room is negative to the surrounding 
area. Access to this room is controlled. 
The fermenters have top mounted drives and all other penetrations are 
through the top of the vessel. The outgases from the fermenters are treated 
to effect removal of moisture prior to passage through a particulate ateri- 
lizable filter. The agitator shaft penetration is fitted with a leak proof 
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