Page 11 of Attachment B 
A scheduled systematic preventive maintenance program for agitator seals, 
control valve, pressure release valves, and equipment/facility safeguards 
should be implemented. Possibly the validation team (with a maintenance 
representative) could manage such a program. 
The efficacy, sensitivity, and accuracy of environmental sampling and 
analytical techniques should be investigated. A sampling protocol docu- 
menting procedures, analysis, sampling points, and sampling schedule 
requirements should also be devised. 
An observation was made of a chemostat, with temporary tubing and fittings, 
sitting on an open laboratory table top in a P-2 laboratory. It is recom- 
mended that all chemostats, operating with recombinant DNA molecules or 
infectious organisms, be set up in class 1 biological safety cabinets or 
with secure leak proof tubing and fittings. 
A registry of all owrkers employed with recombinant DNA processes should 
be established and maintained for reference to determine any abnormal 
medical problems or unusual occurances that someday may be attributed to 
recombinant DNA processes. NIOSH has had considerable experience in 
designing and maintaining such worker registries and can thus, provide 
assistance in design, implementation, and logistical requirements. The 
comparability of individual company registries is desirable for optimum 
and successful surveillance on an industry wide basis. 
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