Page ”4“ of Attachment C 
CONSIST OF IMMOBILIZED ENZYMES, PREPARED ON A RELATIVELY SMALL 
SCALE USING GENETICALLY ENGINEERED ORGANISMS, The ETHYLENE TO 
ETHYLENE OXIDE STEP (THE ONE USING THE IMMOBILIZED ENZYMES) 
WOULD BE CARRIED OUT AT LOW TEMPERATURES AND PRESSURES, The 
SECOND STEP, FROM ETHYLENE OXIDE TO ETHYLENE GLYCOL, WOULD BE 
IDENTICAL TO THE ONE USED IN THE PETROCHEMICAL PROCESS, 
Precautions Taken 
1. Petrochemical - 
Reactions such as these, run at high temperatures and 
PRESSURES, HAVE BEEN SAFELY HANDLED FOR MANY YEARS. THE USE OF 
HEAVY CONSTRUCTION MATERIALS, FREE OF CORROSION PROBLEMS, 
FREQUENT EQUIPMENT INSPECTIONS, REDUNDANT CONTROLS, AND EXCELLENT 
OPERATOR TRAINING, HAVE RESULTED IN A VERY LOW ACCIDENT RATE 
IN THE CHEMICAL INDUSTRY FOR THIS TYPE OF OPERATION, 
2. Fermentation - 
Because of the moderate physical conditions involved in 
these fermentations, little chance exists for catastrophic 
accidents, so long as the flammable materials such as ethylene 
are not allowed to escape. Standard procedures have 
BEEN DEVELOPED FOR ANY FERMENTATION OPERATION, AND FURTHER 
PRECAUTIONS ARE APPLIED TO FERMENTATIONS INVOLVING RECOMBINANT 
DNA, EVEN THOUGH THERE MAY BE NO ADDED DANGER. CONTAINMENT FOR 
RECOMBINANT DNA LARGE-SCALE PROCESSES INVOLVE AT LEAST THE 
FOLLOWING: 
[ 519 ] 
