Page 3 of Attachment C 
Genentech started operations in the South San Francisco faciLity in 
1978 and has experienced rapid expansion. On-going construction in 
the facility is trying to meet the demands of this growth. 
Genentech’s organizational structure consists of four scientific di- 
visions. The Nucleic Acid division is involved in gene synthesis and 
organic chemistry research. The division of Molecular Biology con- 
ducts research in cellular cloning, preparation of complementary DNA, 
messenger RNA, expression of plasmids, and the study of genetic 
structure. A third division, Protein Research and Process Develop- 
ment, is concerned with fermentation research and development, pro- 
tein biochemistry research, and total process development. The fourth 
scientific division, Biology, conducts animal studies and designs 
appropriate animal models for testing. 
DESCRIPTION OF THE WORKFORCE 
Currently Genentech employs approximately 80 persons of which about 
68 are involved directly with research and process development. Very 
few personnel are actually designated as operators of fermentation 
systems. Manpower requirements for monitoring fermentation processes 
and systems are anticipated to be no more than 10-157„ of the total 
workforce. There is no • structured work schedule requirements for most 
employees; most work is conducted during regular daytime hours. Two 
employees currently work a night shift. 
DESCRIPTION OF THE PROCESS 
Process development for the production of A & B insulin chains, pro- 
insulin, and somatostatin is now underway. Development of fermenta- 
tion and extraction techniques are also being conducted. Production 
processes utilizing recombinant DNA will vary depending on the nature 
of the product desired and the steps required to achieve the product. 
General process descriptions for fusion proteins and direct expres- 
sion techniques are outlined in Diagram 1 and 2 respectively. 
Genentech' s fermentation capacity consists of shake flask, 10 liter, 
and 600-liter fermentation systems. The 10 liter fermentors are glass 
and are located in the main production room with the 600-liter fer- 
mentor. The 10-liter fermentors are inoculated using a shake flask. 
Inoculation of the 600-liter fermentor may be accomplished via shake 
flask or from the 10-liter fermentor by transporting the inoculum in 
a glass flask with an attached needle to introduce the contents of 
the flask into the fermentor through a diaphram port. The 10-liter 
and 600-liter fermentors are loaded with nutrients through continuous 
piping from feed tanks. The feed tanks and lines are a closed system 
that is sterilizable and maintained under positive pressure (about 
10 p.s.i.). One feed tank is filled with a chemical killing agent for 
inactivation of the process for emergency measures. 
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