Tab A - Page 2 
Table of Contents 
Page 
1.0 Introduction and Summary 1 
2.0 Background: Previous Administrative Action 3 
3.0 Purpose and Need 8 
3.1 Purpose of the Proposed Action 8 
3.2 Need for the Proposed Action 9 
4.0 Description of Proposed Action and Alternatives 11 
4. 1 Proposed Action 11 
4.2 Alternatives to the Proposed Action 16 
4.2.1 Approval of Original Proposal (September 17, 1982) 
Without Restriction 16 
4.2.2 Approval of Modified Proposal (March 8, 1S83) 
Without Restriction 16 
4.2.3 Approval of Proposal as Approved by N I H on 
June 1, 1983, With Changes Proposed by the 
Investigators 16 
4.2.4 Withhold Approval 17 
5.0 Environmental Effects 17 
5.1 The Physical Environment 17 
5.2 Proposed Test Organisms 22 
5.2.1 Characteristics of £. s_. syrlnoae and 
herblcola strains 22 
5.2.2 Construction of INA Deletion Mutants 28 
5.2.3 Previous Field Tests with INA Minus Bacteria 29 
5.3 Effects of the Proposed Field Test 30 
5.3.1 Risk Assessment 30 
5.3.2 The Test Plot 34 
5.3.3 Crops and Non-Commercial Plant Species 36 
5.3.4 Local Epiphytic Bacterial Populations 37 
5.3.5 Insect Populations 39 
5.3.6 Animal Populations 41 
5.3.7 Atmosphere and Climate 41 
5.3.8 Scientific and Agricultural Benefits 45 
5.3.9 Worst Case Considerations 46 
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