Tab A - Page 4 
1.0 Introduction and Summary 
This document Is an Environmental Assessment (EA) and Finding Of Mo 
Significant Impact (FONSI) concerning the application, under the National 
Institutes of Health (NIH) Guidelines for Research Involving Recombinant DNA 
Molecules (Guidelines), by Crs. Steven lindow and Nickolas Panopoulos of the 
University of California, Berkeley, to field test certain strains of ice- 
nucl eation-minus bacteria prepared by recombinant DNA techniques to determine 
whether those bacteria prevent frost damage to plants. The finding of no 
significant Impact confirms the finding that the proposed field test presented 
"no significant risk to health or the environment" which NIH previously made 
under the NIH Guidelines and published in the Federal Register . (A) 
References cited in this document are tabulated in Section 7.0 of this 
document. They are available for public inspection at the NIH Office of 
Recombinant ONA Activities (CRCA), Building 31, Room 3B10, National Institutes 
of Health, Bethesda, Maryland 20205, cn weekdays between the hours of 8:30 a.m. 
ar.d 5:00 p.m. 
This Environmental Assessment and Finding Of No Significant Impact has 
been prepared in accordance with: (1) the National Environmental Policy- Act of 
1969, as amended (8); (2) 40 CFR §1501. 3(b) and §1508. 9(b) of the Council on 
Environmental Quality Regulations for Implementing the Procedural Provisions of 
the National Environmental- Policy Act (C); ar.d (3) Part 30, Environmental 
Protection, of the General Administration Manual of the Department of Health 
and Human Services (D). Authority to approve such an EA and FONSI was 
delegated to the Director, NIH, by the Director, Office of Management, United 
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