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States Public Health Service, In his memorandum to PHS Agency Heads, dated June 
2, 1980, on the subject, "Delegation of Authority for Carrying Out 
Responsibilities Under Revised Part 30 of the General Administration Manual, 
Environmental Protection." 
The proposed field test tc be conducted on a small plot at the Tulelake 
Research Station in Tulelake, California, would Involve bacteria, common plant 
epiphytes, made "Ice-nucleatlon-minus" ( INA~ ) by recombinant DNA techniques. 
The bacteria would be sprayed at different times on sixteen 40-foot rows of 
potato, tomato and snapbean plants, to see if they protect the plants from 
frost damage. 
The NIH has assessed the environmental effect of the proposed field test 
and has found that it will not have a significant impact on the environment for 
the following reasons: 
1. The INA bacteria prepared by recombinant DNA techniques will not 
express any foreign proteins and neither the original nor the altered 
bacteria are pathogenic to living creatures (Insects, animals, birds, 
man). The original and altered bacteria to be used in the experiment 
have been shown not to be pathogenic to any of the major crops 
growing in the area. 
2. Although small numbers of the INA" bacteria may be carried away from 
the test site by Insects, birds and the wind, no environmental harm 
will result from this, because: 
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