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monthly Intervals. If appropriately marked strains are not detected by 
standard dilution plating procedures these procedures will be repeated 
following standard enrichment procedures. (I) 
The total number of bacteria released in each application season (fall and 
spring) would not exceed 10 liters of fully grown cultures. It is estimated 
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that 10 liters of culture would contain approximately 5x10 cells. Bacterial 
suspensions applied to plants will contain 10^ - 10® cells/ml or 10^ - 10^ 
cells/liter. The quantity of suspensions applied In the field will be 20 
liters/acre. Only 0.09 acre is needed for each trial of a INA" deletion 
strain. Since P_. s.. syrinoae is ubiquitous, the numbers of bacteria released In 
the experiment would be infinitesimal compared to the numbers of P_. syrinqae 
occurring naturally. (I) 
Application will be by hand spraying using a conventional sprayer or by 
dipping the tubers in a powder. To prevent inhalation of bacterial aerosols 
respirators will be used. The bacteria will be applied to plants not in the 
immediate vicinity of other similar plant species to minimize the chance of 
significant spread of the bacteria out of the treatment area during 
application. Plant parts will not be transported away from the site of 
application. Bacterial cultures will be transported from the Berkeley campus 
to the field station in sealed, leakproof containers. Oilutlons to a larger 
volume will be made on site. (I) 
Although both the original (September 17, 1982) and amended (March 8, 
1983) proposals called for the use of bactericides to eliminate the suspect 
organisms in the unlikely event that the plants developed any Injury 
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