Tab A - Page 20 
Under this alternative the field test would be the same as that approved 
by NIH on June 1, 1983 except that the following restrictions subsequently 
proposed by Or. llndow (Y) would be Incorporated: (1) flagged deletions having 
specific ONA sequences or segments physically linked to deletions In the ice 
nucleation gene would not be used to tag the modified IMA" bacteria for 
purposes of monitoring those bacteria for at least the first year of the field 
tests; and (2) ice nucleation deficient mutants of Erwinia herbicola will not 
be used for at least the first year of the field test. 
4.2.4 Withhold Approval 
Under this alternative NIH would reverse its previous approval of the 
field test. 
5.0 Environmental Effects 
5.1 The Physical Environment 
The proposed test site for the experiment is a portion of the Tulelake 
Research Station, located approximately two miles southeast of Tulelake 
California. (See Appendix B for maps and further description.) The test plots 
occupy approximately one acre on the 100-acre section of the field station and 
will' be buffered by 20 feet of fallow land and a ten-foot road on all sides. 
In addition, there will be a band of untreated plants around the edge of the 
0.4 acre plots to protect the treated plants. 
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