Tab A - Page 35 
example, "self-cloning," rearrangements within an organism of its own DNA, do 
not add new Information, and are likely to have already arisen spontaneously In 
a population. Such experiments have been exempt from most of the requirements 
of the Guidelines since the 1978 revision (section 1 1 1 -0-3 In the current 
Guidelines). Similarly, experiments which involve moving ONA, by recombinant 
CNA techniques, from one organisn to another with which it normally exchanges 
genetic information. Is not likely to create a unique organism, and therefore 
has been exempted from the Guidelines (section III -0-4 In the current 
Guidel Ines) . 
If the probability of one or more of these initial four factors (a-d) Is 
zero, the probability of harm from that particular recombinant becomes zero. 
If all four factors are significantly above zero, then the probability of the 
crganlsm maintaining itself in the environment will be reasonably high, and a 
careful consideration of the environmental effects of that organism should be 
undertaken. An analysis of possible outcomes should consider the interactions 
of the recombinant organism with other organisms in the environment. Including 
possible mechanisms for exchange of ONA, the expression of new and possibly 
harmful products by the organisms, and the potential for competitive growth of 
the organism. 
B. Application of the Method to the Proposed Experiment 
1. Presence of Foreign ONA 
Because the bacteria in this proposed field test represent a single 
deletion of chromosomal information, no functional foreign gene has been added. 
32 
[ 229 ] 
IS! I H LI BRA 
