Tab A - Page 37 
3. Survival of Organisms After Escape, and Establishment In the Environment 
Past experiments and observations show that the organisms may survive for 
a while, although probably not through a full yearly cycle and In low numbers. 
Because the deletion Is carried In chromosomal ONA, there is not much 
likelihood for transfer of the recombinant Information to other organisms. In 
order to become established, the deletion mutants would have to compete 
successfully against much larger numbers of the naturally occurring epiphytic 
bacteria Including INA + and INA’ phenotypes of the same species and other wild 
bacterial species. The INA deletion mutants are unlikely to become established 
because of the preexisting colonization of available plant surfaces by these 
naturally-occurring epiphytic bacteria. 
C. Probable Risk 
The probability of any adverse environmental effect of this field test is 
at or near zero based on assessment of risk using the probability method 
itt 
described above. Although the probable risk is low, this Environmental 
Assessment includes the following detailed discussion of how the proposed 
experiment will affect the environment in which it will be conducted. 
5.3.2 The Test Plot 
The field tests will be conducted on small plots within a 100-acre tract. 
The location of this tract is described In Section 5.1. Identical plots will 
be established for potatoes, snapbeans and tomatoes. Each plot will contain 
nine different treatments which will be applied In a randomized complete block 
design with four replications (see plot diagram and description of treatments 
attached as Appendix C). Of the nine "treatments" used for each plot, numbers 
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