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2-5 will Involve the spraying of the modified INA" bacteria. Other "treatments" 
Include untreated controls (number 1), plants treated only with INA* bacteria 
(number 6), spraying with INA" bacteria mutagenized with chemicals (number 7), 
spraying with a naturally occurring bacterium (£. f 1 uorescens ) (number 8), and 
spraying with bactericides (number 9). Each replication of each treatment will 
include four 40-foot rows with the plants spaced approximately one foot apart. 
Two rows of plants for each treatment would be established In early May and two 
rows would be established in mid July, except, tomato plants would only be 
planted In July to be subject to the fall frost. Since each treatment Is 
replicated four times within the plot, the total area of any one treatment is 
0.044 acres and the size cf the total planted area for each plot Is 0.04 acres. 
In addition to the sixteen rows of treated and controlled plants on each plot, 
an additional three rows of plants will be used on each side of the plot and 
five plants on the end of each row to guard against wind, dust and other 
intrusions into the plot. Twenty feet of cultivated barren soil will surround 
each plot and this barren area will be surrounded by a ten-fcot wide dirt road. 
The INA" deletion mutants will be sprayed on the leaves of the emerging 
plants or applied to seed pieces when planted. If the experiment is 
successful, the INA" bacteria will colonize the plants to the exclusion of 
colonization by the INA* bacteria and protect the plants from frost damage. 
Whether or not this occurs, the INA" bacteria will not hanm the plants, because 
the bacteria are not pathogenic to potato, tomato, or beans, the plants that 
will be used in the field test. 
Some of the modified bacteria may be carried to other parts of the test 
plot by insects, birds, and the wind. This will have no effect on the plot, 
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