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5.4.2 Approval of Modified Proposal Without Restriction 
Under this alternative, the field test would be the same as that approved 
by NIH on June 1, 1983, except that antibiotics would be sprayed on the plants 
to kill the bacteria In the unlikely event that the plants were harmed. In 
addition, plants outside the area of application from which the modified 
bacteria can be isolated would be treated with the antibiotics if It is 
necessary to quickly terminate the experiment. The use of antibiotics was 
judged to be an environmentally invasive procedure compared to burning or 
burying. 
5.4.3 Approval of Proposal as Approved by NIH on June 1 , 
1983, With Changes Proposed by the Investigators 
Under this alternative the full test as approved by NIH on June 1, 1983, 
would be approved with the restrictions proposed by the researchers that are 
described in Section 4.2.3 above. 
Elimination of the "flagged" deletion monitoring method for at least the 
first twelve-month period of the field test will not pose any significant risk 
to the environment or Increase the potential for such a risk. "Flagged" 
deletions would have been advantageous only in the event that large numbers of 
different deletion mutants would be used, by making possible unique Southern 
blot hybridization methods and probes to differentiate the various INA" 
strains. Since only two recombinant DNA constructed INA' deletion strains are 
anticipated to be released at this time, no compelling need exists for the 
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