5 
Dr. Anderson admitted the word "inserted" is ambiguous, but felt the word 
"added" is also ambiguous. He and Dr. Gottesman noted that "added" could also 
refer to ENA which is simply attached to the cells' outer membrane and not 
introduced into the cytoplasm or the nucleus. 
Dr. Gottesman said the word "added" premises less than the word "inserted;" 
for this reason the word "added" might be preferable in certain sections of 
the document since it would apply even if the DNA had not inserted in the 
chrcmoscme. 
The subworking group agreed to substitute the word "added" in Section I-B-l-a-(2), 
in the second sentence of Section I-B-l-a-(4) , in the first sentence of Section 
I-B-l-b, and in Section I-B-4-b. They suggested the word "present" be sub- 
stituted for the word "added" in the first sentence of Section I-B-l-a-(4) . 
Dr. Walters said Dr. Temin had also suggested the second sentence of Section 
I-B-2-b should read as follows : 
"In what percentage of cells does expression occur only fran the added DNA?" 
Dr. Gottesman thought this concept was included in the second sentence of 
Section I-B-2-b. She suggested this sentence could be modified to more clearly 
state this idea and would read as follows: 
"In what percentage of cells does expression fran added DNA occur?" 
The suhworking group agreed to Dr. Gottesman 's suggested modification. 
Dr. Walters said Dr. Temin had also suggested a new sentence be added bo 
Section I-B-2-b as follows: 
"In what percentage of cells does expression occur fran other DNA sequences 
as a result of the added DNA?" 
Dr. Anderson questioned how one would test whether expression fran other genes 
occurs as a result of inserting the vector in the chromosome. He did not think 
it currently feasible to test for such expression. 
The sufcworking group agreed the points to consider should not request information 
on whether other genes are expressed if a vector inserts in the genetic material. 
Dr. Walters then called the attention of the subworking group to the letter 
(Attachment IV) fran Mr. Edward Lee Rogers on behalf of the Foundation on Economic 
Trends . 
Mr. Mitchell said the first comment offered by Mr. Rogers is that frequent 
revisions of the points to consider may be needed as experience is gained in 
this field. Mr. Rogers did not believe the points to consider should assume 
haw soon -or how often revisions may be necessary. 
[ 334 ] 
