Attachment II - Page 2 
2 
III-B-4-a. Recombinant DNA or RNA molecules derived therefrom from any 
source except for greater than two-thirds of a eukaryotic viral genane 
may be transferred to any non-human vertebrate organism and propagated 
under conditions of physical containment comparable to ELI and appropriate 
to the organism under study (2). It is important that the investigator 
demonstrate that the fraction of the viral genome being utilized does 
not lead to productive infecrtion. A USDA permit is required for work 
with Class 5 agents . ( 18 , 20 ) . 
It is proposed that the RAC consider these proposed changes at its meeting 
on January 27, 1986. 
[ 465 ] 
