CONTENTS 
1.0 Introduction and Summary 1 
2.0 Background 3 
3.0 Purpose and Need 12 
3.1 Purpose of the Proposed Action 12 
3.2 Need for the Proposed Action 12 
4.0 Description of Proposed Action and Alternatives 13 
4.1 Proposed Action 13 
4.1.1 Planting 13 
4.1.2 Field Layout 17 
4.1.3 Field Observations 17 
4.2 Alternatives to the Proposed Action 18 
4.2.1 Approval of Original Proposal of May 3, 1983 18 
4.2.2 Approval of Proposal of August 19, 1983 18 
4.2.3 Withhold Approval.- 18 
5.0 Environmental Effects 19 
5.1 The Physical Environment 19 
5.2 Tobacco and Growing of Tobacco in Wisconsin 23 
5.3 Crown Gall.' 27 
5.3.1 Biochemical Genetics of T-DNA 28 
5.3.2 Reproduction of the Plants 31 
5.3.3 Verification of Disease Resistance 32 
5.4 Possible Effects of the Proposed Field Test 33 
5.4.1. Accidental Escape of Genetically Engineered 
Plants from the Test Plot 33 
5.4.2. Escape of Pollen or Seed of the Genetically 
Engineered Plants from the Test Plot 33 
5.4 3 . Excision of the Inserted Recombinant DNA 
from the Genetically Engineered Plants 34 
5.4.4 Alterations in Palatabillty to Insect Pests 36 
5.4.5 Alterations in Palatability to Nematodes 37 
5.4.6 Alterations in Susceptibility to Fungal Diseases 37 
5.4.7 Alterations in Susceptibility to Bacterial Diseases 39 
5.4.8 Alterations in Susceptibility to Virus Attack 40 
5.4.9 t Alterations in "Weediness" (Persistence of Tobacco) 41 
5.4.10 Pilfering from the Experimental Plot 42 
5.5 Effects of Alternatives 43 
5.5.1 Approval of Proposal of May 5, 1983 43 
5.5.2 Approval of Proposal of August 19, 1983 44 
5.5.3 Withhold Approval 44 
5.6 Summary and Conclusions 45 
6.0 Consultation 46 
7.0 References 49 
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