1J> INTRODUCTION AND SUMMARY 
This document is an Environmental Assessment (EA) and Finding of No Significant 
Impact (FONSI) concerning the application, under the National Institutes of Health 
(NIH) Guidelines for Research Involving Recombinant DNA Molecules (NIH 
Guidelines), by Agracetus of Middleton, Wisconsin to field test crown gall 
resistant tobacco prepared by recombinant DNA techniques. 
This Environmental Assessment and Finding of No Significant Impact has been 
prepared in accordance with: (1) the National Environmental Policy Act of 1969, 
as amended; (2) 40 CFR Section 1501.3(b) and Section'1508.9(b) of the Council on 
Environmental Quality Regulations for Implementing the Procedural Provisions of 
the National Environmental Policy Act; and (3) Part 30, Environmental Protection, 
of the General Administration Manual of the Department of Health and Human 
Services. Authority to approve such an EA and FONSI was delegated to the 
Director, NIH, by the Director, Office of Management, United States Public 
Health Service, in his memorandum to PHS Agency Heads, dated June 2, 1980, on 
the subject, "Delegation of Authority for Carrying Out Responsibilities Under 
Revised Part 30 of the General Administration Manual, Environmental 
Protection." 
The original submissions to NIH concerning this field test were made by 
Dr. Winston Brill, Director of Research, Cetus Madison Corporation, Middleton, 
Wisconsin. On August 30, 1984, Dr. Brill wrote to Dr. William Gartland of NIH 
that: "The purpose of this letter is to notify you that as of August 17, 1984 the 
business of Cetus Madison Corporation (CMC) was transferred to a partnership 
having the name 'Agracetus' between a subsidiary of W.R. Grace 6c Co. and CMC. 
Environmental Assessment 
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