The fields will have controlled access and good cultural and pest control 
practices will be used. The crown gall resistant tobacco will be isolated 
from plants of the same species outside of the experimental plot in 
accordance with pollination characteristics (Federal Seed Act; 7 CFR, 
Section 201.76). 
Agracetus' Institutional Biosafety Committee reviewed this proposal which 
included appraisals by scientists and consultants knowledgeable of the 
crops, their production practices, and the local geographic conditions where 
they will be grown. 
Any observation that might indicate that a crown gall resistant tobacco has 
a detrimental effect on other plants, animals, humans or the environment 
will be reported immediately to the Director of Research of Agracetus. 
Any problems caused by genetically engineered plants will be reported 
promptly to the Director of the NIH Office of Recombinant DNA Activities 
and the Chairman of the USDA Recombinant DNA Committee. Members 
of the Agracetus IBC and managers of neighboring fields also will be 
notified. 
If there is any indication that something unusual is being transmitted 
specifically from the genetically engineered crown gall resistant plants to 
neighboring plants, the plants will be dug up and destroyed by autoclaving 
or burning. In that event, the plants in the surrounding area would be 
tested the next growing season to determine if those plants had acquired 
the foreign DNA. The soil in which the genetically engineered plants were 
grown can be fumigated if required. 
Environmental Assessment 
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