Field testing will include the parental cultivar (Havana 425) and 
the genetically engineered crown gall resistant line. All plants 
will be observed and recorded in the plot at least once a week 
during the growing season. To provide critical comparison of the 
two treatments, only single row plots will be used. Details are 
given below: 
1. 
Number of Treatments 
= 
2 
2. 
Field Design 
= 
Randomized complete block 
3. 
Replicates 
= 
12 
4. 
Plot Size: Row Length 
= 
23' 
Rows/Plot 
= 
1 
5. 
Distance Between Rows 
= 
38" 
6. 
Distance Between Plants 
(within a row) 
= 
18" 
7. 
Plants/Row (= Plot) 
= 
12 (plus 2 parental types on 
each end of each row) 
8. 
Fertilizer/Lime 
= 
According to soil test 
9. 
Weed Control 
= 
Pre-transplant herbicide and 
mechanical weeding will be 
used (to keep the test 
completely free from weeds) 
Environmental Assessment 
Page 16 
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