*.1.2 
Field Layout 
Figure 1 details the layout of the field plants for this proposed 
test. The test will have border rows of parental-type tobacco. 
After providing a 10* alley, the test will be surrounded on all sides 
by two rows of parental-type tobacco. In addition, two plants of 
parental type will be planted on both ends of each row. To 
virtually eliminate pollen dispersal, all but five of the plants will 
be harvested prior to flowering. Five plants will be kept in the 
field until maturity for seed collection. Field test personnel will 
bag the flowers of these five plants to prevent dispersal of their 
pollen. The plants will be 3' apart up to 100', and the spacing will 
increase to 5 ' up to 200', and 10' thereafter. Both lines flower 
synchronously in greenhouse trials. 
*.13 Field Observations 
The test will be carefully monitored at all stages (from‘*-leaf 
through maturity) of plant growth of the crown gall resistant and 
crown gall sensitive tobacco. Agracetus agronomists will visit the 
field at least once a week. Agracetus molecular biologists, plant 
pathologists, plant physiologists, and consultants in plant 
pathology, horticulture, and entomology will also monitor the field 
trial. Other University of Wisconsin experts may visit the test 
site, if needed. 
Environmental Assessment 
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