At harvest, data on the crown gall resistant and sensitive tobacco 
will be recorded on leaf yield, plant height, texture and thickness 
of leaves, and other traits. Quantitative data will be subjected to 
analysis of variance, and the statistical significance of the 
character means will be tested. 
More detail on field test protocols is provided in the Field Testing 
Plan submitted to NIH on April 29, 1985. 
4.2 Alternatives to the Proposed Action 
4.2.1 Approval of Original Proposal of May 5, 1983 
Under this alternative the NIH Director would approve field tests 
on cotton, soybean, corn, tomato and potato in addition to 
tobacco. 
4.2.2 Approval of Proposal of August 19, 1983 
Under this alternative, the NIH Director would approve field tests 
on cotton and tomato in addition to tobacco. 
4-2 3 Withhold Approval 
Under this alternative the NIH Director would not approve the 
proposal of Agracetus recommended for approval by the NIH 
Recombinant DNA Advisory Committee at its June 1, 1984, 
meeting. 
Environmental Assessment 
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