Despite the occasional rearrangements in restriction patterns of 
the novel DNA in these plants, there have been no apparent 
morphological changes which would suggest a reversion of the 
altered T-DNA to its ability to form crown gall (this would 
presumably result in gall growth in a localized region of the plant, 
although such a gall would not be capable of passage to subsequent 
generations). Agracetus scientists have observed no deleterious 
effects of the T-DNA presence, but have instead found potential 
benefits. 
533 Verification of Disease Resistance 
Agracetus scientists have found that the plants containing ADH- 
T-DNA are resistant to infection by various Agrobacterium 
strains. In the initial greenhouse experiments, ten mature R1 
plants were selected from the HADH2 plants. Ten control plants 
from the H42 5 line from which the HADH2 plants were derived 
were also selected. All plants were mature and apparently in good 
health. Five plants of the HADH2 and the H42 5 were each 
inoculated with A. tumefaciens strain A208 (containing a wild 
type, gall-forming Ti plasmid), and five of each of the two lines of 
plants were inoculated with a different A. tumefaciens, strain B6- 
806, The inoculations were made on each side of a low portion of 
the stem of each plant. The inoculations were made with 1 ml of 
concentrated bacterial culture to superinfect the plants. The 
plants were thoroughly wounded in and about the inoculation site 
with multiple punctures. After 27 days of continued growth, all 
Environmental Assessment 
Page 32 
[ 502 ] 
