the H425 plants of both groups had developed crown gall growth. 
None of the HADH2 plants of either group had developed any 
perceptible crown gall growth at all. 
5A Possible Effects of the Proposed Field Test 
5 A. 1. Accidental Escape of the Genetically Engineered Plants from the 
Test Plot 
Unlike animals which have the ability to walk and to climb, or 
microorganisms which can be carried by the wind, tobacco plants 
are rooted in soil and thus can not accidentally escape from a test 
site. Therefore effects of this field test will be confined to the 
small test plot. 
5A1 2. Escape of Pollen or Seeds of the Genetically Engineered Plants 
from the Test Plot 
As noted above all but 5 of the plants will be harvested prior to 
flowering. Five plants will be kept in the field until maturity for 
seed collection. Agracetus personnel will bag the flowers of these 
five plants to prevent dispersal of their pollen. Thus no pollen or 
seeds should escape. The area of the field test is over 10 miles 
from any other tobacco. One-fourth mile (80 rods) is considered 
sufficient isolation to prevent uncontrolled pollination in the 
production of certified tobacco seed (15). Nicotiana alata is 
sometimes grown in home flower gardens as an ornamental. It is 
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