such restriction of symptoms to inoculated plants. 
The bacterial aminoglycoside phosphotransferase gene is 
expressed in Agrobacterium but not in the genetically engineered 
plants. The yeast ADH gene is not expressed in Agrobacterium or 
in the genetically engineered plants. If, somehow, the yeast ADH 
gene or the bacterial aminoglycoside phosphotransferase gene 
with or without T-DNA would be integrated into another organism 
it is extremely difficult to imagine how any type of problem might 
occur. 
5AA Alterations in Paiatability to Insect Pests 
Major insect pests of tobacco in Wisconsin are green peach aphids 
and green hornworms (22). Many other insect species do not 
colonize tobacco. Any change in the chemical constituents of the 
plant, however, might theoretically alter the response of insects 
so that the plants would provide an opportunity for their increase 
not possessed by the parent variety. The experimental plot will be 
monitored frequently to detect any abnormal insect populations. 
Approved insecticides will be applied if necessary. Any unusual 
insect populations would be ecologically significant only if the 
experimental tobacco were propagated extensively in the 
environment. This will not be the case and any environmental 
impact should be limited to the test plot. 
Environmental Assessment 
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