15 
Dr. Dean suggested Clostridium acetobulyticum and £L lactis be added to the 
list on the basis of data indicating plasmid exchange. 
Dr. Macrina suggested S_^ nutans which had inadvertently been emitted from 
Appendix A be added to Sublist F of Appendix A and to any list compiled by the 
working group. He said evidence would support the addition of thermophylus , 
S . nu lien , S. durans, mitior , and ferus to the list. He also suggested 
Pediococcus pentasaceus , P. acidilactici and Eb_ darmosus be added to the list. 
Dr. Clewell suggested sobrinus be added. Dr. Dean suggested brevis , 
B. anthracis , and IL_ megatenum be added. He said the organisms from sub list B 
of Appendix A which should be added are IL_ natto, Fb_ atemrrus , B. niger , and 
B. globigii . He pointed out that both B^_ natto and B^ globigii are classified 
as EL_ subtilis . 
Dr. Dean called attention to the letter from Ms. Alice Caddcw of Genencor 
(Attachment IV). Ms. Caddo/ had suggested Bacillus amylosacchariticus be added 
to any list of organisms exchanging DNA with the Bacilli . Dr. Deem supported 
the addition of amylosacchariticus to the list being developed by the working 
group. 
Dr. London said only one-way transfer of DNA from the Streptococci into Lacto- 
bacillus casei has been demonstrated. The working group discussed whether 
evidence of one-way transfer would be adequate for addition of the organism to 
the list. They agreed an argument could be made to RAC to include Lactobacillus 
casei on the list on the basis of one-way transfer of genetic information. 
The working group agreed preposed Appendix C-V, Extrachrcmoscmal Elements of 
Gram Positive Organisms , would read as follows: 
"Recombinant DNA molecules derived entirely from extrachrcmosamal 
elements of the organisms listed below (including shuttle vectors 
constructed from vectors described in Appendix C) and propagated and 
maintained in organisms listed below are exeirpt from these Guidelines. 
Bacillus subtilis 
Bacillus pumilus 
Bacillus licheniformis 
Bacillus thuringiensis 
Bacillus cereus 
Bacillus arnylolique f aci ens 
Bacillus brevis 
Bacillus natto 
Bacillus niger 
Bacillus aterrimus 
Bacillus amylosacchariticus 
Bacillus anthracis 
[546] 
