16 
Bacillus globigii 
Bacillus megaterium 
Staphylococcus aureus 
Staphylococcus epidermidis 
Staphylococcus camosus 
Clostridium acetobutylicum 
Pedioooccus daimosus 
Pediococcus pentosaceus 
Pedioooccus acidilactici 
Listeria grayi 
Listeria murrayi 
Listeria monocytogenes 
Streptococcus pyogenes 
Streptococcus agalactiae 
Streptococcus sanguis 
Streptococcus saliva rious 
Streptococcus cremona 
Streptococcus pneumoniae 
Streptococcus avium 
Streptococcus faecal is 
Streptococcus anginosus 
Streptococcus sobrinus 
Streptococcus lactis 
Streptococcus mutans 
Streptococcus equisimilis 
Strep to ooccus thermophylus 
Streptococcus milleri 
Streptococcus durans 
Streptococcus mitior 
Streptococcus ferus 
Dr. Fox said all the organisms on the list generated by the working group 
represent a coherent evolutionary group with the exception of Clostridia 
acetobutylicum . 
Dr. Thome suggested the first paragraph of Appendix C-IV should be modified. 
That paragraph currently reads as follows: 
"Any asporogenic Bacillus subtilis strain which does not revert to a 
sporeformer with a frequency greater than 10“^ can be used for cloning 
DNA with the exception of those experiments listed below. Indigenous 
Bacillus plasmids and phages whose host-range does not include 
Bacillus cereus or Bacillus anthracis may be used as vectors . " 
Dr. Thome suggested the second sentence of this paragraph be deleted. He 
argued that a great deal of evidence demonstrates plasmid exchange between B. 
anthracis , B. cereus and other gr am-pos itive organisms. Exchange of plasmids 
between these organisms would occur in nature. 
[547] 
