GENEI\>COR 
Attachment IV - Page 1 
IBO Kimball Wav 
Genencor, Inc. South San Francisco. CA 94080 Telephone 415-588-3475 
November 15, 1985 
Dr. Elizabeth Milewski 
Executive Secretary 
Recombinant DNA Advisory 
Committee Working Group 
on Gram Positive Bacteria 
National Institutes of Health 
Building 31, Room 3B10 
Bethesda, Maryland 20892 
Dear Dr. Milewski 
As we discussed today, Genencor has identified several species 
of Bacillus which are indicated in the literature to exchange 
genetic material and which were not listed in the memo from J. 
Polak and R. Novick to the Recombinant DNA Advisory Committee. 
These organisms are listed below and the literature references 
are included for your use. 
Bacillus subtilis- 
var. aterrimu s 
var. natto (or B. natto) 
var. niger (or B. niger ) 
Bacillus polymyxa ATCC 842* 
J. Marmur, et al 
J. Bacteriol (1963 ) 
85,461-467. 
[although there is another literature report that B. 
polymyxa NRRL B367 does not transform B. subtilis (Dubnau, et al. 
Genetics (1965) 54:491-498)] 
Bacillus amylosaccharlticus or Yoneda, Y. , et al 
B. subtilis var. amylosacchariticus J. Bacteriol ( 1974 ) 
120: 1144-1150. 
In addition, there is a report in the literature that 
alkalophilic bacilli exchange genetic material with B. subtilis 
(Takinishi, H.,et al, FEBS Letters ( 1983 )]J>4: 201-204). 
We respectfully request that the Working Group consider adding 
the bacilli listed above to the exchanger list. 
If you have any questions on the enclosed material, please do 
not hesistate to contact me. 
Sincerely, 
0. CLj /xrw 
Alice J. Caddow 
Manager of Regulatory Affairs 
enclosures 
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