Federal Register / Vol. 45, No. 20 / Tuesday, January 29, 1980 / Notices 
6733 
HI-A-2-a. Viruses of Eukaryotes 
(summary given in Table 111; see also 
exception given at asterisk at end of 
Appendix D). 
1 1 1— A— 2— a— ( 1 }— (a). Non transforming 
viruses. 
Ill— A— 2— a— (1)— (a)— (7). Adeno- 
Associated Viruses, Minute Virus of 
Mice. Mouse Adenovirus (Strain FL), 
and Plant Viruses. Pi physical 
containment + and HVl host-vector 
shall be used for DNA recombinants 
produced with (i) the whole viral 
genome, (ii) subgenomic DNA segments,' 
or (iii) purified cDNA copies of viral 
mRNA.(37) 
1 1 1- A-2-a -{ 1 H a H^l • Hepatitis B. 
III-A-2-a-(l)-{a)-(2)-(a). Pi physical 
containment -f an HVl host-vector shall 
be used for purified 9ubgenomic DNA 
segments. [38) 
III— A— 2— a— ( 1)— (a )-{2)-{ 6). P2 physical 
containment + an HV2 host-vector, or 
P3 + HVl, shall be used for DNA 
recombinants produced with the whole 
viral genome or with subgenomic 
segments that have not been purified to 
the extent required in footnote 38. 
1 1 1- A-2-a-( 1 H a H-2)-(c)- P2 physical 
containment + an HVl host and a 
vector certified for use in an HV2 
system, or P3 + HVl, shall be used for 
DNA recombinants derived from 
purified cDNA copies of viral 
mRNA.(37) 
Ill-A-2-a-(l)-(aH3). Other 
Nontransforming Members of Presently 
Classified Viral Families.(36 ) 
IlI-A-2-a-(l)-{a)-{3)-{a). Pi physical 
containment + an HVl host- 
subgenomic DNA(33) segments or (ii) 
purified cDNA copies of viral 
mRNA.(37) 
IlI-A-2-sa-(l}-(a)-(^H^)- Pi physical 
containment + an HVl host and a 
vector certified for use in an HV2 
system shall be used for DNA 
recombinants produced with the whole 
viral genome or with subgenomic 
segments that have not been purified to 
the extent required in footnote 38. 
Ill— A— 2— a— (1 }— (a)— (b). Transforming 
Viruses.(37A ) 
III— A— 2— a— (1 )— (b) — (7). Herpes Saimiri. 
Herpes A teles, and Epstein Barr 
Virus. (39) 
IH— A— 2— a— (1 ) — (b) — ( 1 H°)- Pi physical 
containment + an HVl host-vector shall 
be used for DNA recombinants 
produced with purified nontransforming 
subgenomic DNA segments.(33) 
III-A-2-a-(l)-(b)-(7}-(f>)- P2 physical 
containment + an HVl host and a 
vector certified for use in an HV2 
system, or P3 -|- HVl, shall be used for 
(i) DNA recombinants produced with 
purified subgenomic DNA segments 
containing an entire transforming 
gene(3S) or (ii) purified cDNA copies of 
viral mRNA.(J7) 
1 II— A— 2— a— ( 1 )— ( b )— ( J )— ( cr) . P3 physical 
containment + an HVl host-vector, or 
P2 + HV2, shall be used for DNA 
recombinants produced with the whole 
viral genome or with subgenomic 
segments that have not been purified to 
the extent required in footnote 38. 
Ill— A— 2— a— (1 )— (b) — (^). Other 
Transforming Members of Presently 
Classified Viral Families. (36) 
III— A— 2— a— (1) — (b)— (^) — (o). Pi physical 
containment + an HVl host-vector shall 
be used for DNA recombinants 
produced with purified nontransforming 
subgenomic DNA segments. [38) 
II I— A— 2— a — { 1 )— ( b )— (^) — ( ) - P2 physical 
containment + an HVl host and a 
vector certified for use in an HV2 
system, or P3 + HVl. shall be used for 
(i) DNA recombinants produced with the 
whole viral genome, (ii) subgenomic 
DNA segments containing an entire 
transforming gene, (iii) purified cDNA 
copies of viral mRNA, [37) or (iv) 
subgenomic segments that have not 
been purified to the extent required in 
footnote 38. 
Ill— A— 2— a— (2). DNA Transcripts of 
RNA Viruses. 
Ill— A— 2— a— (2)— (a). Retroviruses. 
Ill— A— 2— a— (2)— (a) — (7). Gibbon Ape. 
Woolly Monkey, Feline Leukemia and 
Feline Sarcoma Viruses.(39). 
lII-A-2-a-(2}-( a H^Ho). Pi physical 
containment + an HVl host-vector shall 
be used for DNA recombinants 
produced with purified nontransforming 
subgenomic DNA segments.(33). 
Ill— A— 2— a— (2)— )a)— (7)— (A). P2 physical 
containment + an HVl host and a 
vector certified for use in an HV2 
system, or P3 + HVl, shall be used for 
DNA recombinants produced with 
purified subgenomic DNA segments [38) 
containing an entire transforming gene. 
Ill— A— 2— a— (2)— (a) — (7)— (e). P2 physical 
containment + an HV2 host-vector, or 
P3 + HVl, shall be used for DNA 
recombinants produced with (i) the 
whole viral genome, (ii) purified cDNA 
copies of viral mRNA, [37) or (iii) 
subgenomic segments that have not 
been purified to the extent required in 
footnote 38. 
Ill— A— 2— a— (2)— (a)— (^). Other Members 
of the Family Retroviridiae.(36) 
IIl-A-2-a-(2)-( a )-(2)-(o). Pi physical 
containment + an HVl host-vector shall 
be used for DNA recombinants 
produced with purified nontransforming 
subgenomic DNA segments. [38). 
Ill— A— 2— a— (2) — (a)— {^)— (i»). P2 physical 
containment -t- an HVl host and a 
vector certified for use in an HV2 
system, or P3 -|- HVl, shall be used for 
DNA recombinants produced with (i) 
subgenomic DNA segments containing 
an entire transforming gene, (ii) the 
whole viral genome, or (iii) purified 
cDNA copies of viral mRNA.(37) or (iv) 
subgenomic segments that have not 
been purified to the extent required in 
footnote 38. 
Ill— A— 2— a— (2)— (b). Negative Strand 
RNA Viruses. Pi physical containment 
+ an HVl host-vector shall be used for 
DNA recombinants produced with (i) 
cDNA copies of the whole genome, (ii) 
subgenomic cDNA segments, or (iii) 
purified cDNA copies of viral 
mRNA. (37) 
III— A— 2— a— (2) — (c). Plus-Strand RNA 
Viruses. 
1 1 1— A— 2— a— ( 2 )— ( c )— ( 7 ) . Types 1 and 2 
Sabin Poliovirus Vaccine Strains and 
Strain 17D (Theiler) of Yellow Fever 
Virus. Pi physical containment + an 
HVl host-vector shall be used for DNA 
recombinants produced with (i) cDNA 
copies of the whole viral genome, (ii) 
subgenomic cDNA segments, or (iii) 
purified cDNA copies of viral 
mRNA. (37) 
III-A-2-a-(2}-(c)-{.2). Other Plus- 
Strand RNA Viruses Belonging to 
Presently Classified Viral Families. (36) 
BILLING CODE 4110-08-M 
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