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Federal Register / Vol. 45, No. 20 / Tuesday, January 29, 1980 / Notices 
Vole rickettsia 
Yellow fever virus, 17 D vaccine strain 
C. Class 3 Agents: 
1. Bacterial Agents 
Actinobacillus mallei ' 
Actinobacillus mallei* 
Bartonella — all species 
Brucella — all species 
Francisella tularensis 
Mycobacterium avium, M. bovis. M. 
tuberculosis 
Pasteurella multocide type B ("buffalo" and 
other foreign virulent strains') 
Pseudomonas pseudomallei ‘ 
Yersenia peslis 
2. Fungal Agents 
Coccidioides immitis 
Histoplasma capsulatum 
Histoplasma capsulatum var. duboisii 
3. Parasitic Agents 
Schistosoma mansoni 
4. Viral, Rickettsial, and Chlamydial Agents 
Alastrim, Smallpox, Monkey pox, and 
Whitepox, when used in vitro 
Arboviruses — all strains except those in 
Class 2 and 4 (Arboviruses indigenous to 
the United States are in Class 3, except 
those listed in Class 2. West Nile and 
Semliki Forest viruses may be classified 
up or down, depending on the conditions 
of use and geographical location of the 
laboratory.) 
Dengue virus, when used for transmission or 
animal inoculation experiments 
Lymphocytic choriomeningitis virus (LCM) 
Psittacosis-Ornithosis-Trachoma group of 
agents 
Rabies street virus, when used in 
inoculations of carnivores (See Class 2) 
Rickettsia — all species except Vole rickettsia 
when used for transmission or animal 
inoculation experiments 
Vesicular stomatitis virus * 
Yellow fever virus— wild, when used in vitro 
D. Class 4 Agents: 
1. Bacterial Agents 
None. 
2. Fungal Agents 
None. 
3. Parasitic Agents 
None. 
4. Viral, Rickettsial, and Chlamydial Agents 
Alastrim, Smallpox, Monkey pox, and 
Whitepox, when used for transmission or 
animal inoculation experiments 
Hemorrhagic fever agents, including Crimean 
hemorrhagic fever (Congo), Junin, and 
Macliupo viruses, and others as yet 
undefined 
Herpesvirus simiae (Monkey B virus) 
Lassa virus 
Marburg virus 
Tick-borne encephalitis virus complex, 
including Russian spring-summer 
encephalitis, Kyasanur forest disease, 
Omsk hemorrhagic fever, and Central 
European encephalitas viruses 
Venesuelan equine encephalits virus, 
epidemic strains, when used for 
* USDA permit also required for import or 
interstate transport. 
transmission or animal inoculation 
experiments 
Yellow fever virus — wild, when used for 
transmission or animal inoculation 
experiments 
II. Classification of Oncogenic Viruses on 
the Basis of Potential Hazard (2). A. Low-Risk 
Oncogenic Viruses: 
Rous Sarcoma 
SV-40 
CELO 
Ad7-SV40 
Polyoma 
Bovine papilloma 
Rat mammary tumor 
Avian Leukosis 
Murine Leukemia 
Murine Sarcoma 
Mouse mammary tumor 
Rat Leukemia 
Hamster Leukemia 
Bovine Leukemia 
Dog Sarcoma 
Mason-Pfizer Monkey Virus 
Marek's 
Guinea Pig Herpes 
Luck6 (Frog) 
Adenovirus 
Shope Fibroma 
Shope Papilloma 
B. Moderate-Risk Oncogenic Viruses: 
Ad2-SV40 
FeLV 
HV Saimiri 
EBV 
SSV-1 
GaLV 
HV ateles 
Yaba 
FeSV 
III. Animal Pathogens (3). 
A. Animal disease organisms which are 
forbidden entry into the United States by 
Law (CDC Class 5 agent): 1. Foot and mouth 
disease virus 
B. Animal disease organisms and vectors 
which are forbidden entry into the United 
States by USDA Policy (CDC Class 5 Agents): 
African horse sickness virus 
African swine fever virus 
Besnoitia besnoiti 
Borna diseas virus 
Bovine infectious petechia fever 
Camel pox virus 
Ephermeral fever virus 
Fowl plague virus 
Goat pox virus 
Hog cholera virus 
Louping ill virus 
Lumpy skin disease virus 
Nairobi sheep disease virus 
Newcastle disease virus (Asiatic strains) 
Mycoplasma mycoides (contagious bovine 
pleuropneumonia) 
Mycoplasma agalactiae (contagious agalactia 
of sheep) 
Rickettsia ruminatium (heart water) 
Rift valley fever virus 
Rinderpest virus 
Sheep pox virus 
Swine vesicular disease virus 
Teschen disease virus 
Trypanosoma vivax (Nagana) 
Trypanosoma evansi 
Theileria parva (East Coast fever) 
[40] 
Theileria annulata 
Theileria lawrencei 
Theileria bovis 
Theileria bird 
Vesicular exanthema virus 
Wesselsbron disease virus 
Zyonema farciminosum (pseudofarcy) 
References 
1. Classification of Etiologic Agents on the 
Basis of Hazard. (4th Edition, July 1974). 
U.S. Department of Health, Education, 
and Welfare, Public Health Service, 
Center for Disease Control, Office of 
Biosafety, Atlanta, Georgia 30333. 
, 2. National Cancer Institute Safety Standards 
for Research Involving Oncogenic 
Viruses (October 1974). U.S. Department 
of Health, Education, and Welfare 
Publication No. (NIH) 75-790. 
3. U.S. Department of Agriculture, Animal 
and Plant Health Inspection Service. 
Appendix C 
Section I-E-5 states that exempt from these 
Guidelines are "Other classes of recombinant 
DNA molecules, if the Director, NIH, with 
advice of the Recombinant DNA Advisory 
Committee, after appropriate notice and 
opportunity for public comment, finds that 
they do not present a significant risk to 
health or the environment. (See Section IV- 
E— 1— b — (1)— (d).) Certain classes are exempt as 
of publication of these Revised Guidelines. 
The list is in Appendix C.” 
Under exemption I-E-5 of these Revised 
Guidelines are those recombinant DNA 
molecules that are propaged and maintained 
in cells in tissue culture and that are derived 
entirely from non-viral components (that is, 
no component is derived from a eukaryotic 
virus). 
Appendix D 
As noted above at the beginning of Section 
III— A, certain HVl and HV2 host-vector 
systems are assigned containment levels as 
specified in the subsections of Section III— A. 
Those so classified as of publication of these 
Revised Guidelines are listed below. 
*HV1 — Unmodified laboratory strains of 
Saccharomyces cerevisiae 
‘HVl — The following specified strains of 
Neurospora crassa which have been 
modified to prevent aerial dispersion: (1) 
ini (inositolless) strains 37102, 37401, 
46316, 64001 and 89601. 
(2) csp-1 strain UCLA37 and csp-2 strains FS 
590, UCLA101 (these are conidial 
separation mutants). 
(3) eas strain UCLA191 (an “easily wettable" 
mutant). 
HVl — Asporogenic mutant derivatives of B. 
subtilis 
These derivatives must not revert to 
sporeformers with a frequency greater 
than 10“ data confirming this 
requirement must be presented to NIH 
for certification. The following plasmids 
are accepted as the vector components of 
"These follow the assigned containment levels as 
specified in the subsections of Section III— A with 
one exception. This exception is that experiments 
involving complete genomes of eukaryotic viruses 
will require P3 + HV1 or P2 + HV2 rather than the 
levels given in the subsections of Section III— A. 
