FEBRUARY 15-16 - MINUTES OF MEETING 
9 
D. EQUIVALENCE OF LOWER EUKARYOTE HV SYSTEMS WITH THOSE OF E. COL I . 
1. Equivalency Except for Viruses. 
The RAC considered a proposal for the equivalence of lower eukaryote 
HV systems with those of E. coli . The proposal appeared in the 
January 15, 1979 Federal Register as follows: 
"Equivalence of lower eukaryote HV systems with those of 
E. coli . In accord with Section III-B-1, host-vector 
systems which have been approved as HV1 systems may be 
used under P2 containment conditions for shotgun exper- 
iments with phages, plasmids, and DNA from nonpathogenic 
prokaryotes which do not produce polypeptide toxins. [34] 
For other classes of recombinant DNA experiments with 
these HVl systems, except for the cloning of complete 
genomes of eukaryote viruses, the S. cerevisiae and 
N. crassa HVl systems and S. cerevisiae HV2 systems may 
be used at the physical containment levels applicable to 
EKl and EK2 systems, respectively. Experiments involving 
complete genomes of class 1 eukaryote viruses will require 
P3 + HV2 containment levels. Other eukaryote viruses 
are to be handled on a case-by-case basis. [45]" 
This section contained a typograpnical error as it appeared 
in the Federal Register of January 15, 1979. The next to 
last sentence in the paragraph should have read: 
"Experiments involving complete genomes of class 1 
eukaryote viruses will require P3 + HVl or P2 + HV2 
containment levels." 
The RAC decided to treat this proposal in two sections. Accordingly, 
the RAC first considered the following part of the proposal: 
"Equivalence of lower eukaryote HV systems with those of 
E . coli . In accord with Section III-B-1, host-vector 
systems which have been approved as HVl systems may be 
used under P2 containment conditions for shotgun experi- 
ments with phages, plasmids, and DNA from nonpathogenic 
prokaryotes which do not produce polypeptide toxins. [34] 
For other classes of recombinant CNA experiments with these 
HVl systems, except for the cloning of complete genomes of 
eukaryote viruses, the S. cerevisiae and N. crassa HVl 
systems and S. cerevisiae HV2 systems may be used at the 
physical containment levels applicable to EKl and EK2 
systems, respectively." 
[ 55 ] 
