FEBRUARY 15-16 - MINUTES OF MEETING 
16 
The RAC voted 19 to 1 to accept the proposal as amended by 
Dr. Williams: 
! 'In those cases where genetic exchange has not been demon- 
strated between two bacterial species A and B, neither 
of which is known to be pathogenic for man, animals, or 
plants, recombinant DNA experiments involving only A and 
B can be conducted under P3 containment. [2A] " 
VIII. REQUESTED EXEMPTION UNDER I-E-4 . 
The RAC reviewed a request (628) from Dr. Bruce Carlton of the University 
of Georgia for inclusion of additional Bacillus species on the list pro- 
posed for exemption I-E-4. Dr. Carlton is particularly interested in 
having Bacillus megaterium included on the list. Two members agreed that 
the data are poor and that substantial evidence for exchange is lacking. 
The RAC unanimously voted that more data would have to provided. 
IX. PROPOSED EXEMPTION OF STREPTOMYCES SPECIES THAT EXCHANGE GENETIC 
INFORMATION . 
The RAC considered the following proposal: 
" Streptomyces species that have been shown to exchange chromo- 
somal DNA will be included under the exemption category of 
Section I-E-4 of the 1978 Guidelines. Any recombinant ENA 
molecules that are composed entirely of ENA segments from 
the following pairs of species listed below and to be propa- 
gated within the pair are exempt from the Guidelines. 
S. aureofaciens x S. rimosus 
_S. aureofaciens x S. coeli color 
jS. grisues x S. coel icolor 
S. grisues x £. cyaneus 
S_. grisues x S. venezuelae 
S. lividans x S. coelicolor 
£3. rimosus x S_. coel icolor 
S. parvulus x S. coelicolor 
£>. fradiae x £5. bikiniensis " 
During the 30-day period for public comment, no comments were received. 
It was noted that the proposed exemption is for specific pairs of organisms 
rather than for any combination of the species on the list. Heterokaryosis 
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