FEBRUARY i5-)6 - MINUTES OF MEETING 
17 
is being preposed for some of the pairs as evidence for genetic exchange. 
There was concern by several members about the use of heterokaryosis as a 
criterion for exenange. Three menbers noted that heterokaryons can be set 
up between widely different species. Dr. Parkinson stated that there was 
disagreement about the criteria, and moved to adjourn the meeting. The 
motion failed for lack of a second. 
Dr. Gottesman again reviewed the criteria which have previously been accepted 
as evidence for genetic exchange. There was seme question as to hew hetero- 
Karyosis fits in with the previously accepted criteria. Dr. Gottesman 
said that heterokaryon formation is being equated with F' formation, i.e., 
a plasmid carrying a chromosomal gene. The latter has been accepted as a 
criterion for exchange. Dr. Day stated that there is an intrensic difference 
De tween F' and heterokaryon formation. He stated that he would like to 
see evidence that heterokaryosis leads to a reassortment of genetic markers. 
Dr. Day also noted that the RAC did not have an expert consultant on Streptomyces . 
Dr. Campbell stated that heterokaryon formation frequently leads to recom- 
bination, but Dr. Novick stated that there are cases in which it does not 
lead to recombination. 
Dr. Campbell moved that the proposed list be broken down into two lists. 
There was no second for the motion. 
Dr. Kutter moved that the proposal be tabled until the second day of the 
meeting. In discussing the motion, !)r. Krimsky again noted that there 
were no Streptomyces experts in the room. Dr. Gottesman noted the RAC 
has not had an opportunity to review the primary data. The vote on 
Dr. Kutter' s motion was 5 in favor, 12 opposed. 
Dr. Bedford moved that the proposal be tabled until the next meeting. 
The motion failed by a vote of 5 in favor, 12 opposed. 
Dr. Baltimore moved that the list of Streptomyces species for exemption 
I-E-4 should be the following non-pair-wise list of species: 
S. aureofaciens 
S. rimosus 
S. coeli color 
S. grisues 
S. cyaneus 
S. venezuelae 
The motion has the effect of removing the pair-wise constraint of the 
original proposal and removing certain species. The RAC voted 14 in 
favor, 2 opposed, with 3 abstentions for Dr. Baltimore's motion. 
Executive Secretary's Note : This recommendation was not accepted by 
Dr. Fredrickson and will be referred back to the RAC at its next meeting. 
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